Safranin O
Safranin O Stain
Safranin O or basic red 2, Safranin o stain is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a Gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules.Features of Safranin-o, Safranine-o, Safranin o stain are as under :
| CAS No. | 477-73-6 |
| C.I.No. | 50240 |
| Chemical Formula | C20H19ClN4 |
| Molecular Weight | 350.85 |
| Dye Content | ~95% |
| Absorption Max (EtOH 50%) | 530 - 534 nm |
| Absorptivity (1%,1cm) | > 1350 (on dry basis) |
| Loss On Drying (110° c) | 8% max |