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Safranin O

Safranin O

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Safranin O Stain

Safranin O or basic red 2, Safranin o  stain is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a Gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules.

Features of Safranin-o, Safranine-o, Safranin o stain are as under :

CAS No.

477-73-6

C.I.No.

50240

Chemical Formula

C20H19ClN4

Molecular Weight

350.85

Dye Content

~95%

Absorption Max (EtOH 50%)

530 - 534 nm

Absorptivity (1%,1cm)

> 1350 (on dry basis)

Loss On Drying (110° c)

8% max